HM-CWF4 Full-Wavelength Ultra-Micro Spectrophotometer with Fluorescence Detection

Product Introduction

The HM-CWF4 represents the most capable configuration in the HM ultra-micro spectrophotometer series, integrating full-wavelength UV-Vis spectroscopy (190–850 nm), OD600 bacterial culture monitoring, and a 470 nm excitation fluorescence detection module into one comprehensive bench-top instrument. This combination enables laboratories to consolidate multiple analytical workflows onto a single platform — from routine nucleic acid purity assessment and protein quantification to ultra-sensitive fluorescence-based DNA/RNA detection and full-spectrum colorimetric assays.

The fluorescence module extends the effective detection range for nucleic acids far below the UV absorbance threshold. By binding fluorescent dyes specifically to target molecules, the HM-CWF4 achieves a nucleic acid detection limit of 0.5 pg/µL (dsDNA), with fluorescence linearity R²>0.995, repeatability <1.5%, and stability <1.5%. This level of sensitivity is critical for applications such as next-generation sequencing library quantification, single-cell genomics, and cell-free DNA analysis where sample concentrations are often below 1 ng/µL.

Complementing the fluorescence capability, the full-wavelength scanning function (190–850 nm) enables wavelength sweep measurements, spectral library comparisons, and custom colorimetric protocols that cannot be performed on fixed-wavelength instruments. All detection modes share the same liquid column surface tension platform (0.5–2 µL, no dilution, no cuvette) and three-light-path motor control system, ensuring consistent analytical performance across all measurement types. The integrated Android 10 OS, 7-inch HD touchscreen, built-in thermal printer, and 7000 mAh battery make the HM-CWF4 a self-sufficient analytical workstation for both laboratory and field environments.

Applications / Testing Items

• Ultra-sensitive fluorescence nucleic acid quantification (dsDNA, RNA) using fluorescent dyes, detection limit 0.5 pg/µL

• Full-spectrum UV-Vis wavelength scanning (190–850 nm) for spectral characterization

• Standard UV nucleic acid quantification and purity analysis (A260/A280, A260/A230)

• Protein concentration measurement: UV absorbance (BSA), colorimetric (BCA, Bradford, Coomassie)

• OD600 bacterial culture concentration monitoring

• NGS and third-generation sequencing library preparation and quality control

• Single-cell genomics and low-input DNA/RNA workflows

• Cell-free DNA quantification in liquid biopsy applications

• Custom wavelength-based analyte quantification across UV and visible range

Functional Performance / Features

1. Full wavelength range 190–850 nm + fluorescence (470 nm excitation): The only CWF model combining full-spectrum UV-Vis scanning with fluorescence detection — covers every measurement mode in one instrument.

2. Fluorescence nucleic acid detection (470 nm excitation): Achieves detection limit of 0.5 pg/µL (dsDNA) via fluorescent dye binding; linearity R²>0.995, repeatability <1.5%, stability <1.5%.

3. OD600 bacterial culture module: Dedicated cuvette pathway for bacterial and culture media concentration monitoring; OD600 range 0–4 Abs, stability ≤1%, repeatability ≤1%.

4. Colorimetric Method module across full spectrum: Supports BCA, Bradford, Coomassie, and custom dye-based protocols using any wavelength within the 190–850 nm range.

5. Custom wavelength selection for specialized analytes: Research flexibility to define measurement points for molecules beyond standard nucleic acid and protein panels.

6. Three-light-path automatic switching (0.04 / 0.2 / 1.0 mm): Motor-controlled optical path selection across all wavelengths; total measurable range 0.04–300 A equivalent.

7. No-dilution, no-consumable sample platform: Liquid column surface tension technology enables direct 0.5–2 µL measurement, eliminating sample preparation overhead.

8. High-stability pulsed xenon lamp (PP value <0.5%): Broadband light source for consistent UV-Vis and fluorescence excitation baseline.

9. UV-enhanced CMOS linear array detector: Absorbance precision 0.003 Abs, accuracy ±2% (0.76 Abs @ 260 nm); full-spectrum coverage with high signal uniformity.

10. Detection time <6 seconds: All detection modes — UV, fluorescence, OD600, full-scan — completed rapidly for high-throughput environments.

11. Android 10, 7-inch HD touchscreen, 10,000–100,000 record storage: Complete data lifecycle management with USB export, Wi-Fi/Ethernet upload, and remote software upgrade.

12. 7000 mAh battery (21.6 V), 8-hour runtime: Full operational independence from mains power for flexible deployment.

Main Parameters

Technical Parameters

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