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Product Details
Product Introduction
The HM-CWF2 Ultra-Micro Spectrophotometer extends the capabilities of three-wavelength UV detection with the addition of a fluorescence nucleic acid quantification module, making it a versatile mid-range platform for biological sample analysis. Alongside the standard 230 nm, 260 nm, and 280 nm absorbance channels and OD600 bacterial culture measurement, the HM-CWF2 incorporates a 470 nm excitation fluorescence channel that dramatically increases detection sensitivity for DNA and RNA.
The fluorescence module works by specifically binding fluorescent dyes to target nucleic acids, reducing the detection limit to 0.5 pg/µL (dsDNA) — far below the threshold achievable by UV absorbance alone. This makes the HM-CWF2 particularly valuable for applications where samples are highly diluted, contaminated with interfering substances, or available only in trace quantities, such as NGS library preparation and single-cell genomics workflows.
All measurement modes share the same liquid column surface tension platform — no cuvettes, no dilution, just 0.5–2 µL per test. The intelligent Android 10 interface automatically recognizes the selected detection method and presents results directly on the 7-inch touchscreen, with simultaneous thermal printing and wireless cloud data upload. The three-light-path mechanism (0.04 mm / 0.2 mm / 1.0 mm) and UV-enhanced CMOS detector ensure equally robust performance across UV and OD600 channels.
Applications / Testing Items
UV absorbance-based nucleic acid quantification (230/260/280 nm)
Fluorescence-based ultra-sensitive nucleic acid quantification using fluorescent dyes (dsDNA, RNA)
Nucleic acid purity ratio analysis: A260/A280, A260/A230
Protein concentration determination by UV absorbance (BSA standard)
OD600 bacterial and microbial culture monitoring
NGS (next-generation sequencing) library quality control before sequencing
Single-cell and low-input genomics sample preparation
Trace nucleic acid detection in biopharmaceutical QC workflows
Functional Performance / Features
Integrated fluorescence detection module (470 nm excitation): Enables ultra-sensitive nucleic acid quantification with detection limit down to 0.5 pg/µL (dsDNA) by fluorescent dye binding, linearity R²>0.995, repeatability <1.5%, stability <1.5%.
Three-wavelength UV detection (230/260/280 nm) + OD600: Covers standard nucleic acid purity ratios, protein UV quantification, and bacterial culture concentration in a single instrument.
Dual-mode quantification on one platform: Switch between UV absorbance and fluorescence detection without reconfiguration, maximizing instrument utilization across diverse assay types.
No-dilution, no-consumable measurement: Surface tension technology enables direct 0.5–2 µL loading; no cuvettes or capillaries required.
Three-light-path motor control (0.04 / 0.2 / 1.0 mm): Automatic optical path selection extends measurable absorbance range to 0.04–300 A equivalent, covering both low and high concentration samples.
High-stability pulsed xenon lamp (PP value <0.5%): Consistent light source intensity for reproducible UV and fluorescence baseline performance.
UV-enhanced CMOS linear array detector: Absorbance precision 0.003 Abs, accuracy ±2% (0.76 Abs @ 260 nm).
Detection time <6 seconds: Rapid throughput for both UV and fluorescence assays without compromising accuracy.
Android 10, 7-inch HD touchscreen: Dedicated app modules for Nucleic Acid Test, Fluorescence Test, Protein Test, OD600, UV-Vis, and Colorimetric Method.
Wi-Fi + Ethernet multi-mode upload: Batch data export via USB and over-the-air software upgrades ensure long-term instrument maintenance efficiency.
Built-in thermal printer + 7000 mAh battery: 8-hour untethered operation with on-site printed reports for field and bench-top use.
Main Parameters
| Parameter | Specification |
|---|---|
| Model | HM-CWF2 |
| Detection Module | Ultra-micro nucleic acid & protein detection, OD600 bacterial solution detection, fluorescent nucleic acid detection |
| Wavelength Range | 230 nm, 260 nm, 280 nm (three fixed wavelengths) |
| Fluorescence Excitation | 470 nm |
| Sample Volume | 0.5–2 µL |
| Nucleic Acid Testing Range | 2–15,000 ng/µL (dsDNA) |
| Protein Detection Range | 0.05–400 mg/mL (BSA) |
| Detection Time | <6 s |
| OD600 Absorbance Range | 0–4 Abs |
Technical Parameters
| Parameter | Specification |
|---|---|
| Optical Path | 0.2 mm, 0.04 mm (high concentration); 1.0 mm (standard concentration) |
| Light Source | Xenon flashlight (pulsed xenon lamp) |
| Detector | UV-enhanced CMOS linear array sensor |
| Absorbance Precision | 0.003 Abs (0.2 mm optical path) |
| Absorbance Accuracy | ±2% (0.76 Abs @ 260 nm) |
| Absorbance Range | 0.04–300 A (equivalent to 10 mm optical path) |
| OD600 Absorbance Stability | ≤1% |
| OD600 Absorbance Repeatability | ≤1% |
| Fluorescence Detection Linearity | R²>0.995 |
| Fluorescence Detection Repeatability | <1.5% |
| Fluorescence Detection Stability | <1.5% |
| Operating System | Android 10 |
| Display | 7-inch HD color touchscreen |
| Connectivity | Wi-Fi, Ethernet, USB |
| Data Storage | 10,000–100,000 entries, searchable, exportable via USB |
| Power Adapter | 45 W fast charging |
| Battery Capacity | 21.6 V / 7000 mAh |
| Power Consumption | 25 W (standby: 10 W) |
| Size & Weight | 325 mm × 227 mm × 211 mm; 5.15 kg |
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