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Product Details
Product Overview
The HM Instruments HM--96C Chemiluminescence Microplate Reader is an advanced benchtop analytical instrument engineered for high-precision, low-background quantitative analysis. Utilizing dedicated glow-type chemiluminescence detection technology, the instrument measures sustained, stable photon emission resulting from specific enzymatic catalytic reactions without requiring an external excitation light source, thereby intrinsically avoiding autofluorescence and scattered light background interference.
Equipped with an imported photon-counting photomultiplier tube (PMT) detector and high-precision optical filters (standard 470 nm and 560 nm with customizable wavelengths), the HM--96C achieves an ultra-low detection limit down to 50 pg/mL across a wide spectral range of 230 to 700 nm. Built-in linear plate shaking, chamber incubation from ambient up to 45°C, and support for both 96-well and 384-well microplates make the HM--96C an indispensable core instrument for academic research institutions, molecular biology laboratories, pharmaceutical screening centers, and analytical testing facilities.
Key Features
- Glow Chemiluminescence Technology: Measures sustained photon release from enzyme-catalyzed reactions without excitation light, completely avoiding autofluorescence and stray background light.
- Imported Photon-Counting PMT Detector: High-sensitivity photomultiplier tube directly captures discrete photon events to achieve a low detection limit of 50 pg/mL and a 7-log dynamic range.
- Wide Spectral Range with User-Changeable Filters: Broad optical coverage from 230 to 700 nm with standard 470 nm and 560 nm filter modules, supporting wavelength customization for diverse luminescent substrates.
- Ultra-Low Inter-Well Crosstalk: Precision optical isolation design limits signal crosstalk between adjacent wells to ≤0.01% for 96-well plates and ≤0.05% for 384-well plates, preventing false-positive bleed-through.
- Dual Microplate Compatibility: Seamlessly accommodates both 96-well plates (50–200 μL/well) and 384-well high-density microplates (20–50 μL/well) for versatile throughput requirements.
- Flexible Reading Modes: Offers top-reading end-point measurement and time-resolved kinetic curve tracking to evaluate reaction rates and binding dynamics.
- Built-in Incubation and Linear Shaking: Integrated thermal regulation from ambient to 45°C ensures temperature stability for enzyme kinetics, combined with linear orbital shaking for thorough reagent mixing.
- Intelligent PC Software and Workflow Builder: Comprehensive Windows 10/11 analytical software provides customizable workflow protocols, multi-model curve fitting, and automated tabular data export.
Applications
- Biological Research: Quantitative total protein determination, specific enzyme activity kinetics, gene expression reporter assays (e.g., luciferase assays), and cell viability testing.
- Pharmaceutical Development: High-throughput drug candidate screening, enzyme inhibition assays, pharmacokinetic profiling, and pharmaceutical purity evaluation.
- Clinical and Diagnostic Testing: Immunoassays for tumor markers, infectious disease antigens and antibodies, metabolic hormone profiling, and early pathology biomarker screening.
- Agricultural and Veterinary Medicine: Rapid screening of animal infectious diseases, veterinary drug residue monitoring, and crop pathogen diagnostic assays.
- Food Safety Inspection: High-sensitivity detection of pathogenic microorganisms, food allergens, illegal chemical additives, and mycotoxins in food products and raw ingredients.
- Environmental Monitoring: Trace quantification of organic pollutants, toxic residues, and environmental endocrine-disrupting chemicals in water bodies and soil extracts.
Technical Specifications
| Parameter | Specification |
|---|---|
| Wavelength Range | 230 ~ 700 nm |
| Standard Optical Filters | 470 nm, 560 nm (Replaceable modules, custom wavelengths supported) |
| Detector Type | Imported photon-counting photomultiplier tube (PMT) |
| Detection Sensitivity | 50 pg/mL |
| Dynamic Range | 7 logs |
| Inter-Well Crosstalk | ≤ 0.01% (96-well plate), ≤ 0.05% (384-well plate) |
| Compatible Microplates | 96-well microplate, 384-well microplate |
| Recommended Sample Volume | 96-well plate: 50 ~ 200 μL/well; 384-well plate: 20 ~ 50 μL/well |
| Reading Modes | End-point and kinetic reading (top reading) |
| Temperature Control Range | Ambient ~ 45°C |
| Plate Shaking Mode | Linear shaking |
| Software Operating System | Windows 10 / 11 |
| Power Supply | AC 220 V ± 10%, 50 Hz |
| Operating Environment | Temperature: 5 ~ 30°C; Relative Humidity: ≤ 70% |
| Storage Environment | Temperature: 5 ~ 40°C; Relative Humidity: ≤ 80% |
| Dimensions (L × W × H) | 352 × 256 × 214 mm |
| Net Instrument Weight | 8.4 kg |
Product Enquiries
Contact our sales representative for product enquiries, configuration information and coordination of service requests.
Sales Contact: Kyra
Email: Kyra@hmdzkj.cn
Tel / WhatsApp / WeChat: +86 13792642016
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