Pigeon DNA identification instrument

Overview:

Generally speaking, male pigeons have ZZ chromosomes, while female pigeons have ZW chromosomes. The gender of pigeons is usually not directly determined by their visual appearance, as they do not have obvious gender characteristics. However, by extracting DNA samples from pigeons through genetic testing, genetic testing can be performed to determine their gender. This method utilizes the sex chromosome differences of pigeons. By detecting the presence or absence of specific gene fragments, the gender of pigeons can be determined.

Basic structure of the instrument:

The pigeon sex detector is an instrument for real-time detection of reactions, mainly composed of gene amplification thermal cycle system, fluorescence real-time detection system, microcircuit control system, computer and application software. Two core functional modules are the thermal cycling system and the fluorescence real-time detection system. The working principle of the gene amplification thermal cycle system is basically the same as that of traditional gene amplification instruments, using semiconductor heating and cooling to complete the thermal cycle process. The fluorescence detection system mainly consists of fluorescence excitation components, light signal transmission components, fluorescence detection components, and control systems.

Equipment parameters:

External dimensions: 235mm * 385mm * 175mm (width * depth * height)

Weight: 5.6kg

Electrical parameters:~220V/50Hz, 255W

Data interface: USB 2.0 * 2 (two on the right)

Environmental parameters:

Operating conditions: Temperature: 10-30 ℃, Humidity: 20%~80%

Transportation and storage conditions: temperature: -20~55 ℃, humidity: 20%~80%

Altitude:<2500 meters

Noise level: A-weighted,<60dB

Sample parameters:

Sample size: 16 * 0.2mL

Test tube type: single tube, eight row, 16 well test tube

Sample volume: 15-100uL

Temperature characteristics:

Heating/cooling method: semiconductor heating/cooling

Temperature range: 4 ℃ -99 ℃

Maximum heating rate: ≥ 3.5 ℃/s

Average heating rate: ≥ 2.5 ℃/s

Maximum cooling rate: ≥ 3.5 ℃/s

Average cooling rate: ≥ 2.5 ℃/s

Temperature control accuracy: ≤ ± 0.01 ℃

Temperature accuracy: ≤ ± 0.1 ℃

Temperature uniformity: ≤ ± 0.3 ℃

Optical properties:

Fluorescence detection channels: 2 channels

Light emitting device: High brightness LED

Daylighting device: high sensitivity, high signal-to-noise ratio photodiode

Adapting probes or dyes:

First channel: 470/520 FAM, SYBR Green

Second channel: 530/570 HEX, JOE, VIC

Detection sensitivity: 1 copy

Linear detection range: 100-1010 copies

Linear correlation coefficient: ≥ 0.999

Channel crosstalk: No crosstalk

Detection repeatability: ≤ 1.0%

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