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The HM-CL-4 is a multi-mode imaging system designed for laboratories that need both high-sensitivity chemiluminescence detection and routine gel documentation in a single instrument. It integrates UV transillumination, white-light epi-illumination, and cooled CMOS chemiluminescence imaging, enabling researchers to switch between protein blotting and nucleic acid gel workflows without changing equipment.
At its core is the same 9-megapixel cooled CMOS sensor found in the HM-CL-2, cooled to -25 °C for low-noise long-exposure capture. A 590 nm narrow-band filter and F0.95 high-transmittance lens preserve weak chemiluminescent signals, while a 302 nm UV source provides bright, uniform excitation for ethidium bromide and SYBR-stained gels.
The 15.6-inch touchscreen runs an integrated operating system with preset templates for common applications, reducing training time and minimizing setup errors. For guidance on choosing between the HM-CL-2 and HM-CL-4, see our cost-performance analysis.
Applications
- Western Blot protein detection and quantification
- Southern Blot and Northern Blot nucleic acid analysis
- DNA/RNA agarose gel documentation under UV or white light
- Protein SDS-PAGE gel imaging
- Dot blot and slot blot screening
- Enzyme activity and reporter gene assays
Functional Performance
- Triple imaging modes: Chemiluminescence, UV transillumination, and white-light epi-illumination in one enclosure.
- Cooled CMOS sensor: 9 MP resolution with -25 °C cooling for low-noise imaging.
- 16-bit gray depth: 65,536 gray levels support quantitative chemiluminescence analysis.
- High-transmittance optics: F0.95 lens with ≥ 85% transmission across 400–900 nm.
- 590 nm narrow-band filter: Optimized for chemiluminescence emission capture.
- 302 nm UV source: 6–10 W transilluminator with 3000 h lamp life for nucleic acid gels.
- Smart exposure algorithm: Recommends exposure time and flags saturated pixels.
- Flexible binning: 1×1, 2×2, 3×3, 4×4, 6×6, and 8×8 options.
- Touchscreen workflow: 15.6-inch integrated display controls capture and export.
- Multiple interfaces: USB 2.0 × 2, USB 3.0 × 2, and Ethernet for data export.
- Compact footprint: 460 × 470 × 715 mm benchtop design.
- Low power consumption: ≤ 90 W operating power.
Main Parameters
| Parameter | Value |
|---|---|
| Display | 15.6-inch capacitive touchscreen |
| Imaging area | 150 mm × 150 mm effective field of view |
| Camera | 9 MP cooled CMOS, 16-bit |
| Cooling | -25 °C at 22 °C ambient |
| Pixel size | 3.76 μm × 3.76 μm |
| Resolution | 3000 × 3000 |
| Dynamic range | 16,000:1 |
| Lens | F0.95, ≥ 85% transmittance at 400–900 nm |
| Filter | 590 nm narrow-band, bandwidth 10 nm ± 2 nm |
| UV source | 302 nm, 6–10 W, 3000 h life |
| Power | ≤ 90 W |
| Dimensions | 460 × 470 × 715 mm |
| Weight | 36.1 kg |
Technical Parameters
| Item | Specification |
|---|---|
| Monitor | 15.6-inch capacitive touchscreen |
| Shooting field of view | Effective field 150 mm × 150 mm for blot/membrane/gel |
| Camera | 9 MP; effective area 11.28 mm × 11.28 mm; dark current < 0.0005 e-/p/s; 16-bit (65,536 gray levels); cooling -25 °C at 22 °C ambient |
| Pixel size | 3.76 μm × 3.76 μm |
| Image resolution | 3000 × 3000 (9 MP) |
| Binning | 1×1, 2×2, 3×3, 4×4, 6×6, 8×8 |
| Lens | Aperture F0.95; transmittance ≥ 85% |
| Filter | 590 nm narrow-band filter, bandwidth 10 nm ± 2 nm |
| White light source | LED uniform light strip, symmetrically distributed |
| UV transilluminator | 302 nm wavelength, 6–10 W single tube, 3000 h life, ballast efficiency > 80% |
| External computer | Windows 10; 6th-gen i5 mainboard; 16 GB RAM; 256 GB SSD |
| External interfaces | USB 2.0 × 2, USB 3.0 × 2, Ethernet × 1 |
| Operating voltage | AC 220V 50Hz |
| Power | ≤ 90 W |
| Dimensions | 460 mm × 470 mm × 715 mm |
| Weight | 36.1 kg |
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