Program-controlled quantitative sealing machine

Overview

The program-controlled quantitative sealing machine is electrically driven by an intelligent circuit, with a dedicated internal heating roller, which is specially used for the sealing of 51-well quantitative plates and 97-well quantitative plates. It is used in conjunction with microbial detection reagents to provide simple, fast, and accurate quantitative detection of total coliform bacteria, Escherichia coli, fecal (heat-resistant) coliform bacteria, enterococci, and Pseudomonas aeruginosa. The 51-well quantitative plate and 97-well quantitative plate are semi-automatic quantitative methods designed based on the maximum probable number (MPN) statistical model of the traditional method, which can quantitatively detect water sample results. This method can replace and is superior to the traditional (membrane, and multi-tube fermentation) method for detecting microorganisms in water.

Scope of application:

Used for rapid detection of Pseudomonas aeruginosa, enterococci, total coliform bacteria, fecal coliforms, Escherichia coli, and total colony count in water samples. It can be carried outdoors, used for emergency and quantitative detection.

Implementation standards:

Comply with "GB 3838-2002 Surface Water Environmental Quality Standard"

Comply with "CJ/T 206-2005 Urban Water Supply Quality Standard"

Comply with "GB/T 5750.12-2023 Drinking Water Standard Test Method"

Comply with "CJ/T51-2018 Urban Sewage Water Quality Standard Test Method Heat-resistant Coliform Determination Enzyme Substrate Method"

Comply with "HJ 1001-2018 National Environmental Protection Standard Water Quality Determination of Total Coliform Group, Fecal Coliform Group and Escherichia Coli"

Product description:

The enzyme substrate method detection device consists of four parts: program-controlled quantitative sealing machine, 51 or 97-hole quantitative detection plate, 100mL quantitative bottle, enzyme substrate method detection reagent. The detection range of water samples: drinking water, source water, bottled water, reclaimed water, secondary water supply, pipe network water, wastewater, food water, livestock water, medical water, etc.

Advantages and characteristics:

The enzyme substrate method is a standard method for detecting E. coli included in GB5750-2006. The enzyme substrate method is currently the most advanced method for detecting E. coli in water. It is currently being recognized by domestic testing departments for its convenience, low false positives, and applicability to rapid testing of a large number of samples.

Compared with the multi-tube fermentation and membrane filter methods, the enzyme substrate method has greatly reduced detection steps and has low requirements for the experimental environment. The detection time can be reduced to 24 hours. It has a good application prospect in daily water sample monitoring and emergency monitoring. It can detect and prevent in time and minimize the probability of major public safety accidents. Take the determination of total coliform group in GB5750-2006 as an example.

Method advantages:

1. No need to operate in a sterile room.

2. The manual operation time is less than 1 minute.

3. No need to prepare culture medium and sterilize a large number of glassware.

4. Qualitative and quantitative analysis can be completed in 24 hours without verification test.

The MMO-MUG culture medium that complies with the fixed substrate technology enzyme substrate method of GB5750-2006 "Standard Test Method for Drinking Water" is equipped with a program-controlled quantitative sealing machine, and the test water sample is encapsulated with (51-well quantitative plate) or (97-well quantitative plate).

Working PrincipleAdd water samples containing total coliform bacteria, and the target bacteria are cultured in Minimal Medium ONPG-MUG culture medium at 36℃±1℃. The specific biological enzyme β-galactosidase produced by total coliform bacteria can decompose the color source substrate ONPG in Minimal Medium ONPG-MUG culture medium, making the culture appear yellow; at the same time, Escherichia coli in the water sample produces specific β-glucuronidase to decompose the fluorescent substrate MUG in Minimal Medium ONPG-MUG culture medium, producing characteristic fluorescence. By the same principle, heat-resistant coliform bacteria (fecal coliform bacteria) will decompose the color source substrate ONPG in Minimal Medium ONPG-MUG culture medium when cultured at 44.5℃, making the culture medium appear yellow.

Technical Parameters

Used for GB5750-2006, HJ1001-2018 enzyme substrate method to detect total coliform group, Escherichia coli, fecal coliform group, etc. in water quality.

Reliability: no leakage, no holes.

Stability: can detect more than 50,000 samples, service life is more than 5 years, with ISO9001 quality certification.

Convenience: on/off and backspace keys, quantitative disk counting, automatic energy saving function, cleaning window, error prompt function.

One-touch drainage function.

Large LCD display window, 4 buttons.

No need for sterile room, 24h detection of total coliform bacteria, Escherichia coli and thermotolerant coliform bacteria in water.

Preheating time ≤3min

Noise <48dba

External cover temperature <40ºC

Sealing speed 10 seconds

Working voltage AC 220V±10%, 50HZ

Sealing speed 51-hole, 97-hole quantitative detection plate sealing time ≤12 seconds/piece

Working environment temperature -10ºC-50ºC

Detection range: With 51-hole quantitative detection plate detection range 0-200MPN/100ml (water sample is not diluted)

With 97-hole quantitative detection plate detection range 0-2419MPN/100ml (water sample is not diluted)

Dimensions 408mm long X 340mm wide X 300mm high

Weight 12kg

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