Program-controlled quantitative sealing machine

Overview:

Program-controlled quantitative sealing machine is a special to meet the modern packaging production, driven by intelligent circuit electricity, internal special heating rollers, specifically for 51-hole quantitative tray and 97-hole quantitative tray sealing film use, with microbiological testing reagents to use, to provide a simple, rapid and accurate quantitative detection of total coliforms, fecal (heat-resistant) coliforms, E. coli experimental program; 51-hole quantitative tray and 97-hole quantitative tray It is a semi-automatic quantitative method based on the statistical model of the maximum possible number (MPN) of traditional methods, which can quantitatively detect the results of water samples, and this method can be an alternative to and better than the traditional (filter membranes, and multi-tube fermentation) methods of detecting microorganisms in water.

Application：

Used in water samples of total coliforms, fecal (heat-resistant) coliforms, Escherichia coli rapid detection, field portable, emergency, quantitative detection.

Standards:

Comply with GB 3838-2002 Environmental Quality Standard for Surface Water.

In line with the “CJ/T 206-2005 urban water supply water quality standards”.

Conforms to “GB/T 5750.12-2023 standard test methods for drinking water”.

Conforms to “CJ/T51-2018 Standard Test Methods for Urban Sewage Water Quality Heat-resistant Coliform Determination Enzyme Substrate Method

Conforms to “HJ 1001-2018 National Environmental Protection Standard Determination of Total Coliforms, Fecal Coliforms and Escherichia coli in Water”.

Product description:

Enzyme substrate detection device consists of program-controlled quantitative sealing machine, 51 or 97 holes quantitative detection disk, 100mL quantitative bottle, enzyme substrate detection reagents, four parts, the detection of water samples range: drinking water, source water, bottled water, water, secondary water supply, pipeline network water, wastewater, food water, animal husbandry water, medical water and so on.

Advantageous features:

Enzyme substrate method for GB5750-2006 included in the standard method for the detection of E. coli, enzyme substrate method is currently the most advanced method for the detection of E. coli in water, is now convenient and quick, low false positive, applicable to a large number of samples for rapid detection of the advantages of the domestic testing department is gradually recognized.

Relative to the multi-tube fermentation and membrane method, the enzyme substrate method greatly reduces the number of detection steps, and the requirements of the experimental environment is not high, the detection time can be reduced to 24 hours, in the daily monitoring of water samples and emergency monitoring has a very good prospect of application, can be detected in a timely manner, to prevent and minimize the chances of major public safety accidents, GB5750-2006 in the determination of total coliforms as an example to compare the three methods, as shown in the table. The total coliforms in GB5750-2006, for example, is compared with three methods, as shown in the table.

| Project/Method      | Multi-tube Fermentation | Membrane Method | Enzyme Substrate Method | | Detection Time                        | 3-5 days        | 2-3 days               | 1 day            | | False Positive Rate                 | —                  | 23-26%                 | 0.01            | | Detection Range                    | 2-1600 MPN/100mL    | —                | 1-2419.6 MPN/100mL | | Environmental Requirements | Clean Laboratory     | Clean Laboratory   | No Special Requirements | | Personnel Requirements | Professional Foundation, Skilled | Professional Foundation, Skilled | Simple Training Required | | Quantitative Standard             | MPN Table | Direct Counting            | MPN Table       | | Detection Method                    | 15 tubes        | Colony Counting      | 51 or 97 well plate |

Advantages of the method:

1. No need to operate in a sterile room.

2. Manual operation time is less than 1 minute.

3. No need for medium preparation and sterilization of large quantities of glassware.

4. Qualitative and quantitative analysis can be completed in 24 hours without verification test.

In line with GB5750-2006 “Standard Test Methods for Drinking Water” fixed substrate technology enzyme substrate method of MMO-MUG medium supporting the use of program-controlled quantitative sealing machine, testing water samples with (51-hole quantitative disk) or (97-hole quantitative disk) encapsulation.

Stay [working principle] add the water sample containing total coliform bacteria, the target bacteria in Minimal Medium ONPG-MUG medium at 36 ℃ ± 1 ℃ culture, total coliform bacteria produce specific biological enzyme β galactosidase can decompose Minimal Medium ONPG-MUG medium in the color source of the substrate ONPG, so that the culture shows yellow; At the same time, the Escherichia coli bacteria in the water sample can be detected in the water sample by using the program-controlled quantitative sealer. At the same time, the specific β-glucuronidase produced by Escherichia coli in water samples breaks down the fluorescent substrate MUG in Minimal Medium ONPG-MUG medium and produces characteristic fluorescence. In the same principle, heat-resistant coliforms (fecal coliforms) will decompose the color substrate ONPG in Minimal Medium ONPG-MUG medium when incubated at 44.5℃, making the medium yellow.

Product features:

High efficiency and energy saving: optimized heating element layout and temperature control algorithm, which not only improves the working efficiency, but also reduces the energy consumption.

Easy to operate: equipped with an intuitive and easy-to-understand touch screen interface, users can easily set the working parameters; at the same time, it provides self-diagnostic fault function, which is convenient for daily maintenance.

Safe and reliable: with overload protection, short-circuit protection and many other safety measures.

Technical parameters:

Usage: Used in GB5750-2006 enzyme substrate method to detect total coliforms, Escherichia coli, fecal coliforms and so on.

Reliability: no liquid leakage, no broken holes

Stability: more than 50,000 samples can be detected, the service life is more than 5 years.

Convenience: backspace key, quantitative disk counting, automatic energy-saving function, one-key drainage function, large liquid crystal display window, cleaning window, error alert function.

Quickness: no need for aseptic chamber, 24h detection of total coliforms Escherichia coli fecal coliforms in water.

Weight: 14.2kg

Size: L42cm×W41cm×H33cm

Preheating time: ≤3min

Noise: <48dba

Temperature of outer cover：<40℃

Sealing speed: 10 seconds

Working voltage: AC 220V±10% 50HZ

Working efficiency: 51 holes, 97 holes quantitative detection disk sealing time ≤ 12 seconds / a

Working environment temperature: -10 ℃ ~ 50 ℃

Detection range: with 51-hole quantitative detection disk detection range 0-200MPN/100ml (water samples are not diluted), with 97-hole quantitative detection disk detection range 0-2419MPN/100ml (water samples are not diluted)

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