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96 well fluorescence quantitative PCR detector

96 well fluorescence quantitative PCR detector

Product Number:

HM-P96

Product Difference:

4-channel 96 well real-time fluorescence quantitative PCR instrument

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Product Overview

The HM-P96 real-time fluorescence quantitative PCR system is mainly used to run real-time fluorescence quantitative PCR experiments. Through fluorescence excitation and collection, the experimental process is monitored in real time. The experimental process data is plotted into fluorescence curves, which are presented in real time on the instrument display interface. The experimental data is fitted and analyzed, and a PDF format experimental report can be generated.

Fast and accurate qualitative and quantitative analysis of target nucleic acids contained in biological samples taken from other animals (such as blood, body fluids, etc.), as well as standard curve, melting curve, genotyping analysis, etc. for special samples.

Basic Principles

The principle of HM-P96 real-time fluorescence quantitative PCR instrument: Polymerase chain reaction (PCR) is a molecular biology technology used to amplify specific DNA fragments. It can be seen as a special DNA amplification in vitro, consisting of several steps such as high-temperature denaturation, low-temperature annealing, and appropriate temperature extension to form a temperature cycle, allowing the target gene to be rapidly amplified. It has strong specificity, high sensitivity, simple operation, and time-saving characteristics, and is a major innovation in gene amplification technology. PCR technology can specifically amplify extremely small amounts of target DNA by millions of times, greatly improving the analysis and detection capabilities of DNA molecules.

Real time fluorescence quantitative PCR technology is a method that adds corresponding fluorescent dyes or fluorescent labeled probes on the basis of conventional PCR. During the PCR reaction process, the entire PCR process is detected in real time through changes in fluorescence signals. The total amount of products after each polymerase chain reaction (PCR) cycle is monitored using fluorescent chemicals, and specific DNA sequences in the test sample are quantitatively analyzed using internal or external reference methods.

Application area 

Basic scientific research

Pathogen detection

Animal epidemic detection

Meat product adulteration

□ Genetically modified testing

Food hygiene and quarantine

Drug development and rational use of drugs

Gene expression

Water monitoring

Product features

Adopting advanced thermoelectric refrigeration systems for higher heating and cooling efficiency

The unique constant current power supply and 6-zone independent temperature control method fully guarantee the stable performance of the system. The light source and path design make fluorescence more realistic and reliable

Independent fluorescent channels, reducing crosstalk between different channels

Comprehensive analysis function combined with custom format report function, meeting the needs of customers in different scenarios. The operation software is simple and intuitive, making it easy to learn and get started

ModelHM-PC96
Sample capacity96 holes *0.2ml
Sample volume20-120卩1
Applicable consumables0.2ml single tube, 8x0.2ml row tube, 96 well plate
Temperature Control Range0-99°C
Gradient temperature control6-module gradient temperature control, with a maximum temperature difference support of 25
Heating rateMaximum 7 ° C/s, average 3.8 ° C/s
Cooling rateMaximum 4.5 ° C/s, average 3.5 ° C/s
Temp Accuracy±0.1 °C
Temperature uniformity±0.1 °C
Detection sensitivityDetectable single copy genes
Dynamic range10-10A10
RepeatabilityCV<3%
Dependent linearity0.99-1
Amplification efficiency99%-101%
Analysis algorithmAmplification curve, standard curve quantification, melting curve, genotyping SNP (optional)
TouchDown functionSupport for increasing and decreasing time and temperature modes
Test kitCustom temperature running program, open fluorescence quantitative PCR reagent
Light sourceFour long-life LED lights with different filters can specifically and efficiently excite fluorescent molecules in different bands
Propagation mediumHigh temperature resistant professional optical fiber
Applicable dyes/probesFAM, SYBR- Green; HEX,JOE,VIC,TET; ROX,Texas-Red; CY5
Dimension410*320*196 mm, 11 kg
Power supply220V
Operating systemStandard external WI and Windows computer (WI N7 system or above), optional WI ND O W tablet all-in-one


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