Product Overview
The HM-P96 real-time fluorescence quantitative PCR system is mainly used to run real-time fluorescence quantitative PCR experiments. Through fluorescence excitation and collection, the experimental process is monitored in real time. The experimental process data is plotted into fluorescence curves, which are presented in real time on the instrument display interface. The experimental data is fitted and analyzed, and a PDF format experimental report can be generated.
Fast and accurate qualitative and quantitative analysis of target nucleic acids contained in biological samples taken from other animals (such as blood, body fluids, etc.), as well as standard curve, melting curve, genotyping analysis, etc. for special samples.
Basic Principles
The principle of HM-P96 real-time fluorescence quantitative PCR instrument: Polymerase chain reaction (PCR) is a molecular biology technology used to amplify specific DNA fragments. It can be seen as a special DNA amplification in vitro, consisting of several steps such as high-temperature denaturation, low-temperature annealing, and appropriate temperature extension to form a temperature cycle, allowing the target gene to be rapidly amplified. It has strong specificity, high sensitivity, simple operation, and time-saving characteristics, and is a major innovation in gene amplification technology. PCR technology can specifically amplify extremely small amounts of target DNA by millions of times, greatly improving the analysis and detection capabilities of DNA molecules.
Real time fluorescence quantitative PCR technology is a method that adds corresponding fluorescent dyes or fluorescent labeled probes on the basis of conventional PCR. During the PCR reaction process, the entire PCR process is detected in real time through changes in fluorescence signals. The total amount of products after each polymerase chain reaction (PCR) cycle is monitored using fluorescent chemicals, and specific DNA sequences in the test sample are quantitatively analyzed using internal or external reference methods.
Application area
Basic scientific research
Pathogen detection
Animal epidemic detection
Meat product adulteration
□ Genetically modified testing
Food hygiene and quarantine
Drug development and rational use of drugs
Gene expression
Water monitoring
Product features
Adopting advanced thermoelectric refrigeration systems for higher heating and cooling efficiency
The unique constant current power supply and 6-zone independent temperature control method fully guarantee the stable performance of the system. The light source and path design make fluorescence more realistic and reliable
Independent fluorescent channels, reducing crosstalk between different channels
Comprehensive analysis function combined with custom format report function, meeting the needs of customers in different scenarios. The operation software is simple and intuitive, making it easy to learn and get started
Model | HM-PC96 |
Sample capacity | 96 holes *0.2ml |
Sample volume | 20-120卩1 |
Applicable consumables | 0.2ml single tube, 8x0.2ml row tube, 96 well plate |
Temperature Control Range | 0-99°C |
Gradient temperature control | 6-module gradient temperature control, with a maximum temperature difference support of 25 |
Heating rate | Maximum 7 ° C/s, average 3.8 ° C/s |
Cooling rate | Maximum 4.5 ° C/s, average 3.5 ° C/s |
Temp Accuracy | ±0.1 °C |
Temperature uniformity | ±0.1 °C |
Detection sensitivity | Detectable single copy genes |
Dynamic range | 10-10A10 |
Repeatability | CV<3% |
Dependent linearity | 0.99-1 |
Amplification efficiency | 99%-101% |
Analysis algorithm | Amplification curve, standard curve quantification, melting curve, genotyping SNP (optional) |
TouchDown function | Support for increasing and decreasing time and temperature modes |
Test kit | Custom temperature running program, open fluorescence quantitative PCR reagent |
Light source | Four long-life LED lights with different filters can specifically and efficiently excite fluorescent molecules in different bands |
Propagation medium | High temperature resistant professional optical fiber |
Applicable dyes/probes | FAM, SYBR- Green; HEX,JOE,VIC,TET; ROX,Texas-Red; CY5 |
Dimension | 410*320*196 mm, 11 kg |
Power supply | 220V |
Operating system | Standard external WI and Windows computer (WI N7 system or above), optional WI ND O W tablet all-in-one |
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