8-well PCR instrument

Product Overview

The HM-P08 real-time fluorescence quantitative PCR system is mainly used to run real-time fluorescence quantitative PCR experiments. Through fluorescence excitation and collection, the experimental process is monitored in real time. The experimental process data is plotted into fluorescence curves, which are presented in real time on the instrument display interface. The experimental data is fitted and analyzed, and a PDF format experimental report can be generated.

Fast and accurate qualitative and quantitative analysis of target nucleic acids contained in biological samples taken from other animals (such as blood, body fluids, etc.), as well as standard curve, melting curve, genotyping analysis, etc. for special samples.

Basic Principles

The principle of HM-P08 real-time fluorescence quantitative PCR instrument: Polymerase chain reaction (PCR) is a molecular biology technology used to amplify specific DNA fragments. It can be seen as a special DNA amplification in vitro, consisting of several steps such as high-temperature denaturation, low-temperature annealing, and appropriate temperature extension to form a temperature cycle, allowing the target gene to be rapidly amplified. It has strong specificity, high sensitivity, simple operation, and time-saving characteristics, and is a major innovation in gene amplification technology. PCR technology can specifically amplify extremely small amounts of target DNA by millions of times, greatly improving the analysis and detection capabilities of DNA molecules.

Real time fluorescence quantitative PCR technology is a method that adds corresponding fluorescent dyes or fluorescent labeled probes on the basis of conventional PCR. During the PCR reaction process, the entire PCR process is detected in real time through changes in fluorescence signals. The total amount of products after each polymerase chain reaction (PCR) cycle is monitored using fluorescent chemicals, and specific DNA sequences in the test sample are quantitatively analyzed using internal or external reference methods.

Basic structure of the instrument

The real-time fluorescence quantitative PCR system is an instrument for real-time detection of reactions, mainly composed of gene amplification thermal cycle system, fluorescence real-time detection system, microcircuit control system, computer and application software. Two core functional modules are the thermal cycling system and the fluorescence real-time detection system. The working principle of the gene amplification thermal cycle system is basically the same as that of traditional gene amplification instruments, using semiconductor heating and cooling to complete the thermal cycle process. The fluorescence detection system mainly consists of fluorescence excitation components, light signal transmission components, fluorescence detection components, and control systems.

Instrument characteristics

1. Small size, light weight, and easy to carry. Easily meet the needs of outdoor experiments.

2. Built in 7-inch high-definition capacitive screen PDA, touch screen operation, simple and fast.

3. Marlow high-quality Peltier refrigeration sheets, combined with the German high-end PT1000 temperature sensor and the temperature control mode of the electric resistance heating compensation edge, have a large heating rate of 7 ℃ and a large cooling rate of 5 ℃, greatly reducing the experimental time.

4. Fast lighting mode for the entire board for 3 seconds to ensure consistency in the hole positions of the experimental results.

5. Simple and intuitive software guidance makes it easy to start detection experiments.

Application field

Basic scientific research

Pathogen detection

Animal epidemic detection

Meat product adulteration

□ Genetically modified testing

Food hygiene and quarantine

Drug development and rational use of drugs

Gene expression

Water monitoring

Technical parameters

Sample capacity: 8x0.2ml, supports 8-way tubing

Applicable consumables: Common transparent PCR consumables, 8x0.2ml tube, 0.2ml single tube

Reaction system: 5-120ul reaction mode system

Heating/cooling module: imported semiconductor thermoelectric module

Temperature control range: 4 ° C-99 ℃

Average temperature rise and fall rate ≥ 2 ° C/second

Temperature control accuracy: ≤ ± 0.1 ° C

Temperature uniformity: ≤ ± 0.2 ° C

Number of temperature control areas: multiple points (2 points)

Gradient number: 0

Gradient temperature range: None

Gradient hole count: None

Excitation light source: maintenance free LED

Excitation wavelength range: 400-700nm

Detection component: imported photodetector

Number of detection channels: Standard 1 channel (FAM), High configuration (optional) (FAM, VIC)

Applicable dyes and probes: FAM/SYBR Green I, VIC/HEX/CY3 (optional), ROX/Texas Red (optional), Cy5, TAMARA (optional)

Software function: Fluorescence quantitative PCR system software; Real time amplification reaction curve function; Display of real-time response curves for specific specimens; Data analysis function; Automatic judgment function for yin and yang results; Graphical display function.

Noise:<45 dB

Screen size: 7 inches (HD)

Touch screen: capacitive

External USB: supports data import and export

Hot cover: automatic pressure regulation

Appearance dimensions: 290 (W) * 308 (L) * 130 (H)

Box size: 75 L * 38 W * 19 H cm

Net weight: approximately 3Kg

• Previous: no more
• Next: 16 well PCR detector